General Information

  • ID:  hor005442
  • Uniprot ID:  P29335
  • Protein name:  Insulin B chain
  • Gene name:  ins
  • Organism:  Amia calva (Bowfin)
  • Family:  insulin family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Amia (genus), Amiidae (family), Amiiformes (order), Holostei (infraclass), Neopterygii (subclass), Actinopteri (class), Actinopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0005975 carbohydrate metabolic process; GO:0006006 glucose metabolic process; GO:0007165 signal transduction
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  AASQHLCGSHLVEALFLVCGESGFFYNPNKS
  • Length:  31(1-31)
  • Propeptide:  AASQHLCGSHLVEALFLVCGESGFFYNPNKSGIVEQCCLKPCTIYEMEKYCN
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P29335-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005442_AF2.pdbhor005442_ESM.pdb

Physical Information

Mass: 386204 Formula: C149H221N39O44S2
Absent amino acids: DIMRTW Common amino acids: LS
pI: 6.48 Basic residues: 3
Polar residues: 12 Hydrophobic residues: 12
Hydrophobicity: 23.55 Boman Index: -1354
Half-Life: 4.4 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 78.71
Instability Index: 3689.68 Extinction Coefficient cystines: 1615
Absorbance 280nm: 53.83

Literature

  • PubMed ID:  2039477
  • Title:  Structure and receptor-binding activity of insulin from a holostean fish, the bowfin (Amia calva).